Journal Name:
Thrombosis and Haemostatis
Article Title:
Rapeseed Oil & Sunflower Oil Diets Enhance Platelet in Vitro Aggregation and Thromboxane Production in Healthy Men when compared with Milk Fat or Habitual Diets
Date Written:
1992
Volume:
67
Number:
Page:
352
Author(s):
Mutanen, M.; Freese, R.; Valsta, L.; Ahola, I.; Ahlstr�m, A.
Article:
Substituting dietary saturated fat (SFA) with vegetable oils rich in polyunsaturated fatty acids (PUFA) is generally recommended for the prevention of CHD. Substituting SFA with monounsaturated fat (MUFA) also has favorable effects on blood cholesterol values. In addition, dietary fats have been shown to be involved in thrombosis via an alteration in platelet function. The effect of vegetable oils on platelet function has been investigated to a much lesser extent than fish oils containing long-chain n-3 fatty acids. The objective of this study was to investigate the effect of diets rich in MUFAs, PUFAs, and SFAs on in vitro platelet aggregation in response to each diet, including canola oil (CO).
Twenty-six normolipidemic men (18-60 yrs of age) participated in this highly controlled trial. The study was performed over three consecutive periods. In the first 14-day period, all subjects were given a diet high in milk fat (fat 36% of energy, SFA 19%, MUFA 11%, PUFA 4%). Subjects were then randomly assigned to consume either a sunflower oil enriched diet high PUFA (group 1 SO; n=13) (fat 38% of energy, SFA 13%, MUFA 10%, PUFA 13%), or a MUFA rich diet enriched in CO (group 2; n=13) (fat 38% of energy, SFA 12%, MUFA 16%, PUFA 8%) for a 24 day period. Following 24 days, groups 1 and 2 randomly crossed over to consume the SO or CO diets in reverse order. Subjects were aware of consuming the MF diet during the first 14 days, but were blind to which diet (CO or SO) they consumed for the following two consecutive 24-day periods. All diets were mixed natural diets with similar cholesterol contents. Blood was drawn following an overnight fast at baseline, and following each three dietary periods for analysis of platelet aggregation and thromboxane production.
Results demonstrated that in vitro platelet aggregation induced by 1, 2, and 3 uM of ADP, and 25 ug/ml collagen was significantly increased following consumption of both oil diets compared to the MF diet. Furthermore, enhancement of platelet aggregation was associated with an increased in-vitro platelet thromboxane production following consumption of both oil diets versus the MF diet (p<0.05 after SO diet; p<0.001 following CO diet).
In this highly controlled study, the results demonstrate that vegetable oil diets increased platelet aggregation in vitro suggesting that sunflower and CO may be proaggregatory. These findings conflict with a number of other studies in which consuming vegetable oils were found to be anti-thrombotic. The authors state that further investigation is clearly necessary to fully elucidate the effect of different vegetable oils on platelet reactivity., , ,
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